Thursday, March 25, 2010

The Valley




Chapter I

There was the constant robotic noise with no organic sounds. Barring the constant hum of the fans in the fume hoods, rumble of the rotary pumps of the mass spectrometers and the cyclic squeek of the liquid chormatographs making injections every 4 minutes, the lab produced no sounds at all at this midnight hour. Bright and almost flooding lights from the select segments in the ceiling reflected from every object in the lab- the shiny corners of the hoods, the black slabs on the benches, the steel housing of the instrumentation and the glass windows which reflected portions of these objects on them. I was engulfed in bright loneliness. The environment has a disorienting feel which at times felt like it was the real early morning hour when the rest of the crew has yet to arrive. A moment later it felt like the late evening when the rest of the staff have gone for the day and are perhaps in the process of dining and preparing to go to bed. Or it could just have been that everyone has gone for lunch and will be back in a moment. That’s when I have to look at the wall clock to reorient my self. The clock had just stuck fifteen past twelve at midnight.

It was time to look at the data that just came off the instrument. The previous day ten monkeys were “sacrificed” that were injected with various amounts of the test pharmaceutical molecule-TX450. The samples from their serum were prepared and loaded into the instrument for data acquisition. At midnight the last of the samples would have been acquired.

I had visited the cage room in the morning where the monkeys were kept. As I ran my eyes over each cage, the animals inside them seemed inconspicuous of what was going to happen to them jumping and making noises, except, it seemed, the one in the 9th cage. In cage 9 was a large ape that constantly stared at me from inside. It was the largest of the animals and looked different from the others with its darker mane and deeper eyes. Its eyes were focused on me all along while I was there. Closing my eyes I could still feel its stare on me. It was as if the animal was studying me and as if it knew our plans and was not afflicted. I was its study animal.

Ah, the midnight disorientation. I shook off my thoughts and sat in front of the computer to look at the data. First as usual I checked the control samples to ensure that my experiment was valid. The control samples are prepared form a pool of the serum obtained from the many animals in the study group. The data from these samples showed the expected large peaks from the house keeping lipids typical of the species of the monkeys in the test group and the internal standards spiked into the serum immediately after drawing the blood. The most dominant peaks were from a set of phospholipids typical of the serum from these monkeys. I then began looking at the individual test samples for these peaks to ensure that the samples were acquired correctly. As I carefully huddled through the data I learnt that they were, except for the sample from cage #9! The data had no peak from these phospholipids typical of the monkey species. It had the correct magnitude of the internal standard peaks. This would typically mean that the sample preparation and analysis was done correctly but the sample did not have the phospholipids I was looking for. But then there is also the other possibility. Gary, the technician who collected the sample could have mistakenly interchanged the tubes with the sample from any of the other animal species in the room, rats, squirrels, dog plasma etc that did not contain this lipid in their serum. I will have to wail till morning to check with him on that. There is, however, one thing I could do while waiting. I could find the bag of blood initially collected from the dying animals from which an aliquote of the serum was transferred to the tubes by Gary and then rerun the experiment. If I do it sooner, I could use the same plate controls and save time. If I wait for Gary, the specified amount of time for the use of controls will pass and I will have to prepare new set of controls. So to save a few hours and efforts and material, I rushed to the animal storage room where the bag of blood is refrigerated. Having been in the room earlier when the animals were still alive, I felt heavy at heart to go back where ten monkeys were bleed to death for science profiteering. I do have 2 pet cats and I do feel for animals. Well, I had accepted long ago that life is a road of contradictions and I would never know absolute wisdom from what I perceive are my duties. I did feel terrible going back to that room after the fact. I tuned the lights on and went straight for the wall where refrigerators are placed. I identified the red rubber bag with the blood of the animal in cage #9 and carried it with me to the lab. I thawed the blood in the bag and began preparing the samples for re analysis. When the samples were ready I injected the extract into the analysis vial and waited for the data to be acquired. As the data appeared on the instrument computer screen I clicked the mouse to look for the peaks.

No phospholipids peaks but the expected internal standard peaks were present!..just as I found it the first time!!

The result confirmed that Gary did not make a mistake of switching the tubes between species-if the blood from the animal in cage 9 was collected in this bag. There were no other bags collected that day. Perhaps the blood was contaminated by something in the bag such that the phospholipids were destroyed for this particular sample. Very unlikely but plausible if I find that a bottle of chloroform or isopropanol may have leached into this bag. Perhaps I could find an empty bottle of non polar solvent at the collection site that could explain this observation.

I rushed back to the animal room to take a closer look. I once again opened the door and turned the lights on. This time I turned to walk towards the empty cages from where the blood collection procedure began. After bleeding the animals to death they were taken to a morgue in the next building for disposal the next day. As I walked toward the empty cages, a glimpse at the cage #9 stopped me still and breathless.

Those eyes were still starring at me!!!

The cage #9 had the animal in it and it was looking at me exactly the same way it did in the morning when I had visited the animal room. The animal was supposed to have been dead. The only difference was that now the cage door was open and I was standing defenseless in front of the beast with my back to the wall.

.....to be continued


Chapter II





The wall behind me must have supported my body from collapsing. I stood there with my eye balls feverishly focussed on the beast. I didnot think I had the courage to blink. I was gasping for air and sweating and looking for any signs of aggression from the beast. It appeared as though the ferocity in the eyes of the beast slowly melted and gave way to a sympathetic yarn. I felt a tiny release of ease from somewhere in my spines. The beast stepped out of the cage with its two limbs first and then the body propelled by the hind legs. Now the beast was fully in the open and still focussed on me.

The clock behind stuck 5 and at the same time the beast turned towars the row of glass windows. At the moment the lab doors flung open. More flouroscent lights were tuned on. Gary stood at the open door studying the beast which was near a glass window. The beast tuned back with those firce eyes pinned on Gary. Gary had pointed a shot gun at the beast that was licenced to be used in the event of dangers in the lab. He must have rememberd some thing from the previous day to have been in the lab so early.

"Hi there.."

Gary let his presence be known to me while looking at the beast who was advancing towards him. I heard a click and a loud thud from Gary's gun. A window pane behind the beast shattered. While I recovered from the blasting noise of the gun shot and the shattering of the window pane, I noticed that the beast had Gary's head in his forlimbs. I almost fainted as the next events unweiled. The beast had Gary's lock of hair in its palm and Gary's body was throbbing on the ground. What was once his head was now a fountain of blood overflowing around his body on the ground. It seemed that his skull was crushed under the palms of the beast. What kept me from crashing down to the floor was my involuntary awareness that the the stare of the beast was on me the whole time. While Gary was struggling in its palms, the beast had its eyes on me. Its body language did not convey that it was watchful of my plans but it felt as if it was revealing something to me. This time I could not notice an iota of sympathy in its stare.

It took a few steps towrds me and then tuned back, with two hops reached the shattered window pane. With its arm it slashed the pane further to make a bigger opening and as if a toy it pushed the netting out of the buidling. In matter of seconds the beast had disappered from the room. The window opened itself up to a patio which faced a conservation land..900 acres of Shanendoah forest and campgrounds. I glimpsed at the possible path of the beast through the window. The moon shone over the valley casting a silhoutte of the large mangroves and the silver river. The early birds had began chirping.

To be continued....

Saturday, December 26, 2009

Are you listening?

As you get off the crowded bus,
Walk into the narrow stony path,
With green grass margins either side,
All you see is shade, struggle and life.

As you foot the twisted path,
Growing sound of the flowing stream,
Will welcome you with impounding coolness.

Soon the stony moist path
Will merge with the busy water stream.
Stepping onto the wooden plank,
You will note the paddy stretch,
Miles and Miles you run your eyes over,
Until it gives way to the fading blue hue of the hills afar.

As you walk the soil barriers crack;
Dividing the vegetation you behold-
Some green, some gray and some in gold,
Dancing in the bright noon sun,
Chances are that you will meet,
A man in seventies on a rock,
Amidst the green his innovation.
He will smile to you as you note,
His eager week but once bright eyes.

He shall keep his shovel aside,
Throw the beedy butt away,
And offer from his earthen pot,
Cool well water to quench your thirst.
He shall lead you to his dwelling,
Culmination of his tireless years.
You shall cross the sweet smelling
Garden he nurtured like his child.
He shall tell you the forgone tales,
Of distant wars and forgotten men.
He may tell you of his past;
Of how as a kid he roamed the umpire,
And how he slept on the curbs of lanes.
He may tell you of his deeds,
Of how he fought for his land
Or how he raised his beloved kids
With little more than sunshine and air.
He wont tell you of that thought,
That he concealed deep in his heart,
Of how he thought he could not let,
His children have their dreams in full-
Of how matter deceived his conviction.

But now o' traveler if you will
Go back to that remote valley some day,
You shall not meet the wrinkled old man
For he no longer sits on the moist rock.

But sure does he see the little earth,
Ans sure does he watch his own garden,
From somewhere in the endless skies.

Saturday, October 24, 2009

A Turtles Life


If you tune your integrated devices to the historical data and search with a key -Heronwood drive- you will find that a marsh pond once existed there in the early 20th century -a hundred years ago where a fusion cell recycling center now stands. I was conceived there and born on its bank. Even a turtle like me can clearly recollect those days when a row of 11 human houses stood arond the marsh pond and lots of kids played, bicycled and patiently followed my mom, I and my brother Joe as she was giving us a tour of the area as a part of my early childhood training. That tour is the last piece of memory I have of my mother. Some where along the tour the amused human kids grabbed me and my brother Joe and held us in their palms. Then they ran into one of the houses and put us in some sort of a box. In the box was some water, rock and a dock and where we met a middle aged turtle from a different sect but who was kind to us babies. He told us that he has been there for several years and just wants to pull on. We just lay there in the water hoping to crawl out when we could. Next day Joe was taken out of the box by a human kid leaving me and the adult turtle in the box. He told me that it could be my turn next and I would end up in a home like he has. Some times living older has its benefits. He was right. I was grabbed, put in a green bucket and taken to another house on the street. With my turtle sense I realized that this is the house on the bank of the marsh pond closest to where I was born. Perhaps I could see my mom again..

It was not to be. I lived there just long enough to be sent to this place I call home - this serene wild life sanctuary. I have lived here for over a hundred years now. Care taker humans have come and gone. Some kind, and some not so kind. My days here are quite simple. I get my food, I have no enemies to fear from and I have fellow turtles to hang out with. I am still young and only a 100 years old.

Now I digress.

Back in the house near the pond there were three humans, two grown ones and one baby like myself. Whenever I stuck my neck out I could see the human baby bending down to look at me upclose and smiling. He talked about keeping me with him for ever and would ask the grown ups to feed me even if they have already done so. I thought to my self that you don’t have to be a turtle baby to be cute- he was so very cute. I took an immediate liking to him.

Would you believe me if I tell you that these days he still visits me almost every year in the sanctuary and spends some time with me? That’s how I keep the memory of my childhood so strong and alive. Over the years I have learnt that he has graduated from college, when he formed his Indy Punk group and when he had his first performance. He seemed so happy and I feel happy for him as well. After all he is the oldest friend I have. Occasionally he would bring his family, his pretty wife, his 2 kids and his cat. They all seemed interested in me, although I am not sure what the intentions of the cat were. Jokes aside, like I have always said, the human babies are as adorable as the turtle ones. The best part was when several years back he brought these twin babies in a stroller, his new grand children. I could not contain my excitement. I crawled as close to the path as I could to catch a glimpse of those sleeping babies. I was overjoyed and even had a drop of tear of joy. They looked so adorable together and for just a fleeting moment I remembered my brother Joe. Does he still live in the area and do he and mom hang out together and think of me?

Cycle of life...that's what it is. Just like my human friend, I have had kids of my own here in the sanctuary. Unlike the human kids they don’t stick to me for too long. I still see them, I watch them play and in them I see Joe and the twin babies of my human friend. Till my friend shows up again some time next year, I will lay here on the rock and take life easy.

Sunday, February 15, 2009

The day I met my knight in Shining Armour..

* this poem is a parody of the message in a Fathers day card my wife gave me. The first lines of each stanza and those in italics are the original lines from the message and is credited to the card manufacturer. The rest is my take.



The day I met my husband - was he a knight in shining armour,

Today he shivers when at night I pick up a hammer.


The day I met my husband -I felt my world had changed,

Today I ensure that the oil in my car – he changed.


I glazed into his eyes and knew we would be together,

To day I stare at him and his knees come together.


A sweet charmer was he-gentle and caring

If today my coffee not so sweet, I fix his bearing.


He continues to bring change and joy to our castle,

When on Wednesdays he clears the trash in joy and dazzle.


Yes, my knight in shining armour was a hulk,

A man above all other men - today he is just a bulk.


Sunday, January 18, 2009

Back to the Craddle

Whenever I had seen pictures of the Grand Canyon with descriptions of its grandeur clogged in superlatives, I have discounted it as a large overrated pit. I had learnt, with amusement reserved only for fiction that the Native Americans believed that the spirits of their ancestors came to their final rest in the ravines of the canyon. One day a few fellow grad students decided to rent a car and drive up to the rim of the pit and I joined in. We drove up north to Flagstaff, AZ a town with a constant cool alpine breeze owing to its altitude, and from there further north up to the south rim of the canyon. We planned to camp in the woods around the rim and hike a modest distance inside the rim the next day. Those days, being a sucker for hiking I was quite happy with the plan.

As we neared the rim, the ambiance began to change. The shapes of the trees were now more twisted and the branches more spiraled. The smell and the sound of the breeze indicated a definite change in the geography. The car was still going up the hill when we began to notice the parked tour buses and people gazing around with cameras. We parked our car and I began walking toward the rim. The edge of the rim was marked by a two feet stone wall over which some children were playing with the rather giant squirrels. People pointed their cameras to various points on the horizon before them. The sign near the bushes read do not feed the squirrels but that didn’t seem to deter some kids who were chasing the squirrels with huge bags of potato chips trying to feed them. I walked up to the wall and took my first look at the “pit”. The moment jolted me in surprise for clearly I did not expect to see what lay before me. Looking up at the skyscrapers in Manhattan or Chicago I had always felt an awe that triggered a minor rush of adrenaline as I would begin to imagine being up on the top and looking down from the roof. Here I was physically on top of a height way beyond what I could have imagined looking down from any skyscrapers. Beneath my foot lay the depths of many skyscrapers, the combined height of the Empire State building and the Seers towers and many more - and I was standing on the roof of that height. The grand depth pointed downwards to a slither of windy twine of the blue almost green Colorado River. Along the walls I could see the hikers moving down or up like ants - disappearing into or emerging from the deep brown dust that glowed in the evening sun.

My preconception of the canyon had now been completely erased.

I looked down at the enormous grandeur that was spread under my feet and my mind left me fixated on to the rim to wander. The draw of the site let my mind go beyond its routine of placing me on the roof of the heights for a simulated excitement. With my eyes lost on the unending layers of the ravine each depicting a time my mind began flashing before me with instincts I had not known since my childhood and as if they had been waiting all these years beneath my own layers to spring. This experience had nothing to do with my stated purpose of the trip. The vastness of the canyon space and walls seemed to re-define the fading profile of my late father’s life through war and peace well before I knew to perceive. Like a breeze the feel of him standing by my side brushed over me. I watched the darkening red walls of the ravine silently encapsulating the vast depths of the canyon. In its gravity, I could touch the senses that never before landed it self to me. The depth below felt as if it is related to the unfed depths of my own. It cradled me as if I had no mass. I felt secure as a child as if my fathers arm was around my shoulders.

I was being handed back my childhood.

The grains of passage of time are carved out on the walls of the canyon with unrelenting clarity. Perhaps all that I have known and felt in my time will too be preserved on the canyon wall as a speck some day. The sun was just setting on the west rim and the edges of the east rim glowed as if it were conversing with the sun. My eyes glazed in the spectacle of its final glow and even after the sun was gone I was left with the remnant of that glow in my pupils for many minutes. A cool breeze hissed in from the alpine woods, touched my shoulders and disappeared somewhere into the darkening ravine beneath my feet – perhaps to trigger waves in the deepest rivulets of the ravine.

I had never felt as fabulously insignificant as I did when walking back to the car.

Tuesday, December 30, 2008